TABLE 2.
Phage infection of E. coli O157:H7 strain 86-24 Nalr and its O-antigen-deficient derivatives
| E. coli O157:H7 straina | Description | Latex agglutination test (O157 antigen) | Plaque
formation
|
Lytic efficiency of phage mixture
atb:
|
||||
|---|---|---|---|---|---|---|---|---|
| KH1 | KH4 | KH5 | 37°C
|
4°Cc | ||||
| Aerated | Nonaerated | |||||||
| 86-24 Nalr | Nalidixic acid-resistant mutant of E. coli O157:H7 strain 86-24 | + | + | + | + | + | − | + |
| F12 | O157 antigen-deficient mutant derived from strain 86-24 Nalr | − | − | − | − | − | − | − |
| F12(pSK+) | F12 transformed with plasmid Bluescript SK+ | − | − | − | − | − | − | − |
| F12(pF12) | F12 transformed with rfbEECO157:H7 cloned into pSK+ | + | − | − | − | − | − | − |