Table 6. The effect of different incubation periods (d) and medium volume per container volume (ml) on productivity of BCs by Streptomyces sp. KB1 (TISTR 2304) against S. aureus TISTR 517 under incubation temperatures of 25 °C, 30 °C, and 35 °C.
Each data point indicates the average antimicrobial activity across various conditions. Antimicrobial activity after culturing at different temperatures.
| Medium volume/container volume (ml) | Mean diameter of inhibition zone (mm) at different incubation period (d)* | ||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | ||||||||||||||||
| 25 °C | |||||||||||||||||||||||||||||
| 50/250 of baffled flask | 0.00 ± 0.00 | 0.00 ± 0.00 | 16.33 ± 0.58 | 17.33 ± 0.58 | 17.67 ± 0.58 | 17.67 ± 0.58 | 17.33 ± 0.58 | 17.33 ± 0.58 | 17.33 ± 0.58 | 17.00 ± 0.00 | 16.33 ± 0.58 | 16.33 ± 0.58 | 16.00 ± 0.00 | 15.67 ± 0.58 | |||||||||||||||
| 100/500 of baffled flask | 0.00 ± 0.00 | 0.00 ± 0.00 | 15.33 ± 0.58 | 15.33 ± 0.58 | 15.67 ± 0.58 | 15.67 ± 0.58 | 15.67 ± 0.58 | 15.00 ± 0.00 | 15.00 ± 0.00 | 14.67 ± 0.58 | 14.67 ± 0.58 | 14.67 ± 0.58 | 14.00 ± 0.00 | 14.00 ± 0.00 | |||||||||||||||
| 200/1,000 of baffled flask | 0.00 ± 0.00 | 0.00 ± 0.00 | 11.33 ± 0.58 | 11.67 ± 0.58 | 11.67 ± 0.58 | 12.33 ± 0.58 | 12.00 ± 0.00 | 12.00 ± 0.00 | 12.00 ± 0.00 | 12.00 ± 0.00 | 11.67 ± 0.58 | 11.67 ± 0.58 | 11.67 ± 0.58 | 11.00 ± 0.00 | |||||||||||||||
| 30 °C | |||||||||||||||||||||||||||||
| 50/250 of baffled flask | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 15.33 ± 0.58 | 16.67 ± 0.58 | 16.67 ± 0.58 | 17.33 ± 0.58 | 17.67 ± 0.58 | 17.33 ± 0.58 | 17.67 ± 0.58 | 17.67 ± 0.58 | 16.67 ± 0.58 | 16.33 ± 0.58 | 16.00 ± 0.00 | |||||||||||||||
| 100/500 of baffled flask | 0.00 ± 0.00 | 18.00 ± 0.00 | 18.67 ± 0.58 | 18.67 ± 0.58 | 18.67 ± 0.58 | 18.67 ± 0.58 | 19.00 ± 0.00 | 19.00 ± 0.00 | 19.00 ± 0.00 | 19.00 ± 0.00 | 18.67 ± 0.58 | 18.67 ± 0.58 | 18.33 ± 0.58 | 18.33 ± 0.58 | |||||||||||||||
| 200/1,000 of baffled flask | 0.00 ± 0.00 | 21.00 ± 1.00 | 22.00 ± 1.00 | 23.67 ± 0.58 | 23.67 ± 0.58 | 23.67 ± 0.58 | 23.33 ± 0.58 | 23.67 ± 0.58 | 23.67 ± 0.58 | 23.33 ± 0.58 | 23.33 ± 0.58 | 23.33 ± 0.58 | 23.33 ± 0.58 | 22.33 ± 0.58 | |||||||||||||||
| 35 °C | |||||||||||||||||||||||||||||
| 50/250 of baffled flask | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | |||||||||||||||
| 100/500 of baffled flask | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | |||||||||||||||
| 200/1,000 of baffled flask | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | |||||||||||||||