FIG. 5.

Accumulation of solutes in H. elongata CHR63. (A) Cells were grown in M63 medium supplemented with different salt concentrations and samples were withdrawn in the late exponential phase. (B) Cells were grown in M63 medium supplemented with 1.0 M NaCl, and samples were taken at different growth stages. Turbidity (OD₆₀₀) values of 0.5 and 1.0 correspond to the early and late exponential phases of growth, respectively, and values of 1.6 and 1.8 correspond to the stationary phase. The solutes were identified and quantified by ¹H NMR by using ethanol cell extracts. The values are the means of values from at least two independent determinations, and the individual values did not differ by more than 20%.