TABLE 3.
List of antibodies
| Antibody | Source/Catalog # | Species | Dilution (WB) | Dilution (IF) |
|---|---|---|---|---|
| COG1 | ABclonal A17594 | Rabbit | 1:1000 | 1:500 |
| COG2 | Sigma OS2 #041212 | Rabbit | 1:1000 | — |
| COG3 | Lab | Mouse | 1:1000 | 1:500 |
| COG4 (C terminal) | Sigma SAB4200469 | Rabbit | 1:1000 | — |
| COG8 (C terminal) | Sigma SAB4200427 | Rabbit | 1:1000 | 1:500 |
| Giantin/GOLGB1 | Covance PRB‐114C | Rabbit | — | 1:2000 |
| GM130/GOLGA2 | CalBiochem, CB1008 | Rabbit | — | 1:2000 |
| GM130/GOLGA2 | BD Biosciences, 610283 | Mouse | 1:400 | |
| Golgin84/GOLGA5 | Warren's Lab | Rabbit | 1:1000 | — |
| Golgin84/GOLGA5 | Warren's Lab | Rabbit | — | 1:1000 |
| P115/USO1 | Sztul's Lab | Rabbit | 1:1000 | 1:400 |
| β‐Actin | Sigma, A5541 | Mouse | 1:2000 | — |
| TGN46/TGON2 | Bio‐Rad AHP500G | Sheep | 1:2000 | 1:500 |
| LAMP2 | DSHB, H4B4 | Mouse | 1:2000 | — |
| TMEM165 | Sigma HPA038299 | Rabbit | 1:2000 | — |
| B4GalT1 | R&D Systems AF‐3609 | Goat | 1:500 | 1:500 |
| Myc‐tag | Cell Signaling 9B11 | Mouse | 1:2000 | 1:2000 |
| Rab6 (C19) | Santa Cruz #SC310 | Rabbit | — | 1:400 |
| Rab2A | Avivoasysbio #QC32060 | Rabbit | 1:1000 | — |
| Rab30A | Thermo Fisher #PA537206 | Rabbit | 1:500 | — |
| Rab1B | ABclonal #A7514 | Rabbit | 1:1000 | 1:400 |
| ERGIC53/LMAN1 | Enzo OTI1A8 | Mouse | 1:1000 | — |
| GS15/BETL1 | Lab | Rabbit | 1:500 | — |
| GS15/ BETL1 | BD Biosciences, 610961 | Mouse | — | 1:300 |
| GS28/GOSR1 | BD Biosciences, 611185 | Mouse | 1:1000 | — |
| Syntaxin5/STX5 | Synaptic Systems, 110‐053 | Rabbit | — | 1:300 |
| YKT6 | ThermoFisher, A305‐479A | Rabbit | 1:1000 | — |
| Syntaxin 5/STX5 | Lab | Rabbit | 1:1000 | — |
| GPP130 | Covance, PRB‐144C | Rabbit | 1:1000 | 1:1000 |
| SDF4/Cab45 | Sigma HPA011249 | Rabbit | 1:1000 | 1:400 |
| GALNT2/GalNacT2 | R&D, AF7507‐SP | Sheep | 1:1000 | 1:500 |
| MGAT1 | Abcam, ab180578 | Rabbit | 1:500 | 1:400 |
| hFut8/FUT8 | R&D, BAF5768 | Sheep | 1:500 | — |
| GGA2 | BD Biosciences #612612 | Mouse | — | 1:400 |
| AP1β1/AP1B1 | Sigma HPA065226 | Rabbit | — | 1:300 |
| β′COP/COPB2 | ABclonal, A7036 | Rabbit | — | 1:400 |
| γCOP/COPG1 | Duden's Lab | Rabbit | — | 1:2000 |
| GOLPH3 | Sigma SAB1102208 | Rabbit | — | 1:500 |
| IRDye 800 anti‐Goat | LiCOR/926‐32214 | Donkey | 1:20 000 | — |
| IRDye 800 anti‐Mouse | LiCOR/5‐32210 | Goat | 1:20 000 | — |
| IRDye 800 anti‐Rabbit | LiCOR/8100901 | Donkey | 1:20 000 | — |
| Alexa Flour 647 anti‐Goat | Jackson Immuno Research/705605‐147 | Donkey | 1:4000 | 1:500 |
| Alexa Fluor 647 anti‐Sheep | Jackson Immuno Research/705605‐147 | Donkey | 1:4000 | 1:500 |
| Alexa Fluor 488 anti‐Rabbit | Jackson Immuno Research/705605‐151 | Donkey | — | 1:500 |
| Alexa Fluor 488 anti‐mouse | Jackson Immuno Research/715‐545‐151 | Donkey | 1:4000 | 1:500 |
| Alexa Fluor 647 anti‐mouse | Jackson Immuno Research/705605‐151 | Donkey | 1:4000 | 1:500 |
| Alexa Fluor 647 anti‐rabbit | Jackson Immuno Research/705605‐152 | Donkey | 1:4000 | 1:500 |