Figure 2.
(A) Comparison of CYP mRNA levels between stably transduced TK6 cells with empty vector (EV) or parental TK6 controls. The Log2 fold change was calculated by ΔΔCt method using GAPDH as the housekeeping gene. (B) Characterization of CYP protein expression in stably transduced TK6 cells. The protein levels of CYP isoforms ranging in molecular weight between 45 and 60 kDa were detected by Western blotting. Myc was used as a tag for transfection and GAPDH (37 kDa) was used as a loading control. Similar results were obtained from three independent experiments.