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. Author manuscript; available in PMC: 2023 Feb 27.
Published in final edited form as: J Vis Exp. 2020 Mar 24;(157):10.3791/59955. doi: 10.3791/59955

Figure 2: Comparison of DNA damage measured in fresh and frozen minced and homogenized mouse liver samples.

Figure 2:

Liver was harvested from two staggered cohorts (n = 5/group/cohort) of male Sprague Dawley rats administered corn oil for 4 days. Slides were prepared from freshly minced tissue, frozen minced tissue, and frozen cubed tissue processed in Merchant’s medium or mincing solution using the tissue mincing device. Frozen tissues were analyzed ~3.5 and 6 months following necropsy for cohorts 1 and 2, respectively. Comets classified as hedgehogs based on morphology were not included in the scoring of % tail DNA. (A) Cohort mean % tail DNA results. (B) Cohort mean % hedgehog results. Error bars reflect standard deviation. Statistical analyses were conducted to compare frozen tissues against fresh tissue for each cohort and to compare the results between the two cohorts for each tissue preparation method. *Statistically different (p < 0.05) from fresh minced tissue within the same cohort using the Student’s t-test; #statistically different (p < 0.05) from fresh minced tissue within the same cohort using the Mann-Whitney test for non-normally distributed data; ¥ statistical difference (p < 0.05) between cohorts using the Student’s t-test.