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PPK characteristics. (a) Arrhenius plot of PPK activity dependence on temperature. (b) PPK activity dependence on magnesium concentration. (c) PPK activity dependence on ATP concentration. Dashed line, best fit to Michaelis-Menten equation (Vmax = 43.4 × 103 U per μg of protein; Km = 2.05 mM); solid line, Michaelis-Menten equation with a Vmax of 28 × 103 U per μg of protein and a Km of 1 mM.
