TABLE 1.
Evaluation of monoclonal antibodies for detecting C. botulinum type B toxin with the ELISA
| Strain | Sourcea | Reactions with monoclonal antibodiesb
|
||||
|---|---|---|---|---|---|---|
| 5BB/25.3 | 5BB/21.3 | 5BB/9.3 | 5BB/4.3 | 3BB/110.3 | ||
| Proteolytic strains | ||||||
| Okrac | CAMR | 3.1 ± 0.9d | 7.4 ± 2.5 | 20.5 ± 11.8 | 104 ± 82 | 6.4 ± 3.1 |
| NCTC 7273 | NCTC | + | + | + | +/− | ND |
| Danish | DSI | + | + | + | + | ND |
| UN180284/1 | CAMR | + | + | + | +/− | ND |
| UN180284/2 | CAMR | + | + | + | + | ND |
| 53B | NFPA | + | + | + | + | ND |
| 1356/B | WB | + | + | + | + | ND |
| NCTC 751 | NCTC | + | + | + | + | ND |
| ATCC 439 | ATCC | + | + | +/− | + | ND |
| NCTC 3807 | NCTC | + | + | + | +/− | ND |
| 115B/1 | NFPA | +/− | + | − | +/− | ND |
| 5009 | WB | + | + | + | +/− | ND |
| 657 | CAMR | + | + | +/− | + | ND |
| B6 | UR | + | + | − | + | ND |
| BL 143 | UR | + | + | + | + | ND |
| BL 86 | UR | + | + | +/− | + | ND |
| BL 150 | UR | + | + | +/− | + | ND |
| BL 152 | UR | + | + | + | + | ND |
| BL 146 | UR | + | + | + | + | ND |
| BL 147 | UR | + | + | + | + | ND |
| NCTC 438 | NCTC | + | + | + | + | ND |
| BL 264 | UR | + | + | + | + | ND |
| BL | UR | + | + | + | + | ND |
| Nonproteolytic strains | ||||||
| ATCC 17844 | ATCC | − | − | − | − | +/− |
| 2B | UR | − | − | − | − | +/− |
| BL 151 | UR | +/− | − | − | − | +/− |
| ATCC 17B | ATCC | − | − | − | − | +/− |
| FT50 | UR | − | − | − | − | +/− |
| CDC 3875 | CDC | +/− | − | − | − | +/− |
| CDC S900A-T-A-3 | CDC | +/− | − | − | − | +/− |
| 2129B | NFPA | +/− | − | − | − | +/− |
| CDC 5900 | CDC | − | − | − | − | +/− |
| KapchunkaB2 | NFPA | − | − | − | − | +/− |
| KapchunkaB5 | NKPA | − | − | − | − | +/− |
CAMR, Centre for Applied Microbiology and Research, Salisbury, United Kingdom; NCTC, National Collection of Type Cultures, London, United Kingdom; DSI, Danish Serum Institute; NFPA, National Food Processors Association, Berkeley, Calif.; WB, Wellcome Biotech, Beckenham, United Kingdom; ATCC, American Type Culture Collection, Rockville, Md.; UR, Unilever Research, Bedford, United Kingdom; CDC, Centers for Disease Control, Atlanta, Ga.
+, toxin detected with sensitivity similar to the sensitivity of control strain detection; +/−, toxin detected with sensitivity lower than the sensitivity of control strain detection; −, toxin not detected; ND, not determined. The values are the amounts of toxin (expressed as nanograms per milliliter) which resulted in optical density values which were 0.3 U greater than the background value.
Control strain.
Mean ± standard deviation (n − 1; n = 11).