TABLE 3.
Comparison of the abilities of the BoNT/B endopeptidase assay and the mouse lethality test to detect crude neurotoxin present in spiked food samples
| BoNT/B concn (pg/ml) | Pate
|
Sausage
|
Buffer
|
|||
|---|---|---|---|---|---|---|
| Endo-peptidase assaya | Mouse test | Endo-peptidase assaya | Mouse test | Endo-peptidase assaya | Mouse test | |
| 50 | 2.78 | + | 2.02 | + | 2.57 | + |
| 25 | 2.80 | + | 1.90 | + | 2.54 | + |
| 12.5 | 2.81 | + | 2.09 | + | 2.69 | + |
| 6.3 | 2.15 | + | 1.82 | − | 1.80 | + |
| 3.1 | 1.24 | − | 1.19 | − | 1.09 | − |
| 1.5 | 0.78 | − | 1.05 | − | 1.02 | − |
| 0 | 0.02 | − | 0.004 | − | 0.04 | − |
a
The endopeptidase assay values are mean absorbance values (n = 4).