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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Nat Chem Biol. 2022 May 19;18(9):934–941. doi: 10.1038/s41589-022-01019-1

Figure 1.

Figure 1.

Benzaldehyde probe 1 reversibly labels cellular proteins. (a) Chemical structures of XO44 and probe 1. (b) Jurkat cells were treated with XO44 or 1 (2 μM, 30 min), followed by compound washout for the indicated times. Cells treated with 1 were lysed in the presence of 25 mM sodium cyanoborohydride, except as indicated (*). After copper-promoted click conjugation with TAMRA-azide, samples were analyzed by in-gel fluorescence and Coomassie blue staining (Supplementary Figure 1). Data are representative of two independent experiments.