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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Nat Chem Biol. 2022 May 19;18(9):934–941. doi: 10.1038/s41589-022-01019-1

Figure 3.

Figure 3.

Quasi-irreversible engagement of AURKA in cells. (a) COS-7 cells were transfected with WT or K162Q (#) Flag-AURKA and treated with probe 3 (30 min). After lysis in the presence of sodium borohydride and TAMRA-azide conjugation, samples were analyzed by in-gel fluorescence and western blotting. (b) Concentration-dependent labeling of Flag-AURKA (~50 kDa TAMRA band, mean ± SD, n=3). (c,d) COS-7 cells were transfected with Flag-AURKA and treated with probe 3 (2 μM, 30 min), followed by washout for the indicated times. Samples were analyzed as described above (mean ± SD, n=3). * and NT indicate non-transfected cells.