Skip to main content
. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Nat Chem Biol. 2022 May 19;18(9):934–941. doi: 10.1038/s41589-022-01019-1

Extended Data Fig. 3. Rapid dissociation of probe 3 from overexpressed SRC.

Extended Data Fig. 3

(a) COS-7 cells were transfected with Flag-SRC, or not transfected (*), and treated with probe 3 (2 μM, 30 min), followed by washout for the indicated times. After lysis in the presence of sodium borohydride and TAMRA-azide conjugation, samples were analyzed by in-gel fluorescence and western blotting. (b) Normalized fluorescence intensity of ~65 kDa band corresponding to Flag-SRC (mean ± SD, n = 3).