TABLE 3.
Effects of electroporation parameters, length of incubation in the presence of 10% glycine, and pH of EPB on the transformation efficiency of S. salivarius ATCC 25975a
| Resistanceb | Voltagec | Length of incubation with glycined | pHe | Transformants/μg |
|---|---|---|---|---|
| − | − | − | − | 7.00 × 103 |
| − | + | − | + | 1.23 × 105 |
| + | − | − | + | 1.32 × 105 |
| + | + | − | − | 0 |
| − | − | + | + | 5.25 × 103 |
| − | + | + | − | 7.63 × 104 |
| + | − | + | − | 9.55 × 104 |
| + | + | + | + | 0 |
| 0 | 0 | 0 | 0 | 6.61 × 104 |
| 0 | 0 | 0 | 0 | 8.05 × 104 |
| 0 | 0 | 0 | 0 | 5.55 × 104 |
a
Cells were grown in HJGS. Glycine was added to cultures at an OD660 of 0.5, and the cultures were incubated for an additional 2 h.
b
−, 100 Ω; 0, 200 Ω; +, 400 Ω.
c
−, 1.4 kV; 0, 1.65 kV; +, 1.9 kV.
d
−, 1 h; 0, 2 h; +, 3 h.
e
−, pH 4.0; 0, pH 4.5; +, pH 5.0.