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. 2023 Jan 23;4(2):222–239. doi: 10.1038/s43018-022-00506-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — (a) nano-LC/MS/MS spectra showing fucosylated HLA-DRB1 peptide (arrow). (b) Flow cytometric analysis for relative cell surface fucosylation (upper) and cell surface HLA-DRB1 levels (upper middle), qRT-PCR analysis of relative HLA-DRB1 mRNA levels (lower middle), and IB analysis of HLA-DRB1 protein levels (lower) in indicated melanoma cell lines treated with DMSO (D), 250 µM FUTi (i), or 250µM L-fuc (LF). n = 3 biologically independent experiments. All error bars represent standard error of the mean.

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