Table 3.
The startup cost included the instruments and equipment prices. The reagent and consumable cost from raw wastewater to extracted RNA ready to be quantified with RT-qPCR or ddPCR.
| Method | Startup Cost ($) | Reagent and Consumable Cost ($ per replicate/sample) | |
|---|---|---|---|
| Manual | Electronegative Membrane with Bead Beating[46] | 15,368 | 7.02 |
| Electronegative Membrane with Elution[46] | 11,160 | 11.32 | |
| PEG[46] | 20,288 | 16.54 | |
| Ultrafiltration[46] | 9000 | 17.62 | |
| Manual ESP | 2874 | 3.81 | |
| Automated | Promega Maxwell RSC[47] | N/A | ∼ 40.00 |
| King Fisher Flex[47] | N/A | ∼ 40.00 | |
| Automated ESP | 25,939 | 3.81 |