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. 2021 Oct 12;25(1):122–132. doi: 10.1007/s11307-021-01657-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — In vivo fluorescence imaging of uIgG-800F, uFab2-800F, and uFab-800F during the optimal imaging window in orthotopic squamous cell OSC-19 tongue tumors: a NIR images taken with the Artemis clinical camera of orthotopic OSC-19 tongue tumors 96 h after uIgG-800F, 48 h after uFab2-800F, and 36 h after uFab-800F administration. Tumors are identified with a yellow arrow. Note the lower exposure time needed to visualize the tumor after uIgG-800F administration compared to uFab2-800F and uFab-800F. b TBRs and c tumor MFIs measured using the Pearl for uIgG-800F, uFab2-800F, and uFab-800F at respectively 96, 48, and 36 h after administration demonstrating similar TBRs between the groups but a higher MFI for uIgG-800F. a.u., arbitrary units; hrs, hours; MFI, mean fluorescence intensity; NIR, near-infrared; ns, not significant; TBR, tumor-to-background ratio; T, time.