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. 2023 Feb 14;14:1117762. doi: 10.3389/fphar.2023.1117762

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Copyright © 2023 Luo, Wang, Zhang, Zeng, Yi, Li, Pan, Zhu, Lin, Liang, Verkhratsky and Nie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The CMIC of CL with U373 cells. (A) The HPLC chromatogram of the standard solution (Polyphyllin VI, Polyphyllin I). (B,C) THe HPLC-MS chromatogram of CL extract. (D,E) The HPLC chromatogram of the eluate of U373 cells and U373 cells expressing P2X₃. (F,G) The HPLC chromatogram of dissociation medium of U373 cells and U373 cells expressing P2X₃.