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. 2021 Nov 15;25(1):3–17. doi: 10.1007/s11307-021-01665-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1. — Comparison of two-photon and confocal microscopies. TPLSM (a,c,e) and CLSM (b,d,g) images of mounted carotid artery, stained for cell nuclei with Syto13. The quality of images is compared in different depths of the sample: at the surface (a,b); 40 µm below (c,d), and 80 µm below (f,g) the surface [1].