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. 2021 Nov 15;25(1):3–17. doi: 10.1007/s11307-021-01665-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6. — TPLSM endoscopic images of internal organs. a Single frame from video of normal colonic mucosa collected in vivo [78]; b image 20 to 30 µm below the surface of the interior colon showing a cross-sectional view of a crypt [79]; c image 45 µm below the surface with crypt (C) and goblet cells (g) [25]. d,e,f Label-free endoscopic images of kidney capsule in a mouse: d TPEF (in red) and SHG (in green) overlay of fibrotic kidney capsule; e,f comparison of collagen structures in fibrotic and healthy kidney [16]. Scale bars = 50 µm. g Redox ratio measurement in mouse kidney in normal conditions, during induced ischemia and reperfusion. Decreased redox ratio is visible in ischemia state [39]. Scale bars = 10 µm.