Fig. 4.

miR-422a and miR-483-5p promote adipogenesis in hBMSCs. A Adipogenesis-related mRNA fold change in hBMSCs transfected with miRNA mimics and negative miRNA mimic controls (CTR− and CTR +). CTR- indicates hBMSCs induced to differentiation in absence of indomethacin, while CTR + with indomethacin. MiRNA mimics were added to the adipogenic medium without indomethacin. * vs CTR−; # vs miR-422a mimic, ° vs miR-483-5p mimic. B Adipogenesis-related mRNA fold change in hBMSCs transfected with miRNA inhibitors. MiRNA inhibitors were added to the complete adipogenic medium. CTR + indicates hBMSCs treated with miRNA inhibitor negative control #1 and induced to adipogenesis with a complete adipogenic medium. * vs CTR + ; # vs miR-422a inhibitor. C Representative images and densitometric quantification of cells staining with Oil Red O. For mimic experiments: * vs CTR−; # vs miR-422a mimic, ° vs miR-483-5p mimic. For inhibitor experiments: * vs CTR + ; # vs miR-422a inhibitor. D Graph chart represents adiponectin released into the culture medium expressed in ng/ml. For mimic experiments: * vs CTR−; # vs miR-422a mimic, ° vs miR-483-5p mimic. For inhibitor experiments: * vs CTR + (E) Osteogenesis-related mRNA fold change in hBMSCs transfected with miRNA mimics and inhibitors of miR-422a and miR-483-5p. * vs CTR + ; # vs miR-422a mimic. Data are mean ± SD of three independent experiments. *, #, ° t-test p < 0.05; **, ##, °° t-test p < 0.01; ***, °°°t-test p < 0.001