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. 2022 Sep 6;46(1):270–284. doi: 10.1007/s10753-022-01732-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — HIF-1α alleviated the TNF-α-mediated apoptosis in MNPCs. a–c The expression levels of Bcl-2, BAX and Caspase-3 were detected using real-time PCR. d–h The protein levels of Bcl-2, BAX and C-Caspase-3 were assayed using Western blotting. i, j TUNEL staining was performed to examine the apoptosis. k Flow cytometry was performed to detect apoptosis. The scale bar was 100 μm. The values were the mean of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.