Table 1.
Antibodies Used for FACS Analysis, Immunostaining, Western Blot Analysis, and In Vivo Studies
| Company | Catalog number | Antibody description |
|---|---|---|
| Thermo | 17-7222-82 | IL-22 Monoclonal Antibody (IL22JOP), APC, eBioscience |
| Fisher Scientific | 50-162-19 | TCR beta, APC-eFluor 780, clone: H57-597, eBioscience |
| Fisher Scientific (BD) | BDB562892 | γδ T-Cell Receptor Hamster anti-Mouse, Brilliant Violet 421, Clone: GL3, BD |
| Fisher Scientific (BD) | BDB563151 | CD4 Rat anti-Mouse, Brilliant Violet 605, Clone: RM4-5, BD |
| Thermo | 25-7133-82 | IL-13 Monoclonal Antibody (eBio13A), PE-Cyanine7, eBioscience |
| Thermo | 45-0441-82 | PerCP-Cyanine5.5 Anti-Human/Mouse CD44 (IM7) |
| Fisher Scientific | BDB552051 | CD4 Rat anti-Mouse, APC-Cy7, Clone: GK1.5, BD |
| Thermo | 67-0081-82 | CD8a Monoclonal Antibody (53-6.7), Super Bright 702, eBioscience |
| Thermo | 45-0149-42 | CD14 Monoclonal Antibody (61D3), PerCP-Cyanine5.5, eBioscience |
| Thermo | 45-0193-82 | CD19 Monoclonal Antibody (eBio1D3 (1D3)), PerCP-Cyanine5.5, eBioscience |
| Thermo | 45-0452-82 | CD45R (B220) Monoclonal Antibody (RA3-6B2), PerCP-Cyanine5.5, eBioscience |
| Thermo | 46571182 | TCR gamma/delta Armenian Hamster anti-Mouse, PerCP-eFluor 710, Clone: eBioGL3 (GL-3, GL3), eBioscience |
| Thermo | 53-7311-82 | IFN gamma Monoclonal Antibody (XMG1.2), Alexa Fluor 488, eBioscience |
| Fisher Scientific | 50-112-3698 | CD8a Rat anti-Mouse, PerCP-Cyanine5.5, Clone: 53-6.7, eBioscience |
| Biolegend | 506914-BL | Anti-IL-17A Rat Monoclonal Antibody (Alexa Fluor 700) [clone: TC11-18H10.1] |
| Fisher Scientific (BD) | BDB742893 | BV510 Rat Anti-Mouse I-A/I-E |
| Thermo | 14-7321-81 | PE TNF alpha Monoclonal Antibody (MP6-XT22), eBioscience |
| Thermo | 53-9966-42 | Gata-3 Monoclonal Antibody (TWAJ), Alexa Fluor 488, eBioscience |
| Thermo | 45-0441-82 | PerCP-Cyanine5.5 Anti-Human/Mouse CD44 (IM7) |
| Thermo | 17-5773-82 | FOXP3 Monoclonal Antibody (FJK-16s), APC, eBioscience |
| BD Biosciences | 560705 | Alexa Fluor 700 Hamster Anti-Mouse TCR β Chain Clone H57-597 (RUO) |
| BD Biosciences | 562894 | BV421 Mouse Anti-Mouse RORγt Clone Q31-378 (RUO) |
| Thermo | 12-5825-82 | T-bet Monoclonal Antibody (eBio4B10 (4B10)), PE, eBioscience |
| Thermo | 45-0031-82 | CD3e Monoclonal Antibody (145-2C11), PerCP-Cyanine5.5, eBioscience |
| Thermo | 45-0112-82 | CD11b Monoclonal Antibody (M1/70), PerCP-Cyanine5.5, eBioscience |
| Fisher Scientific (BD) | 562959 | BV421 Rat Anti-Mouse CD127 Clone SB/199 (RUO) |
| Thermo | 63-5941-82 | NK1.1 Monoclonal Antibody (PK136), Super Bright 600, eBioscience |
| Thermo | 67-5893-80 | KLRG1 Monoclonal Antibody (2F1), Super Bright 702, eBioscience |
| Thermo | 12-6981-82 | ROR gamma (t) Monoclonal Antibody (B2D), PE, eBioscience |
| Novus | NBP1-75516 | IL-33 |
| Invitrogen | PA5-47007 | IL-33 |
| Millipore | 05-1567 | P-120 Catenin |
| Abcam | ab31704 | DCLK1 |
| Research Resource Center UIC and Pocono Rabbit Farm | DRA∗ | |
| Sigma | G9545 | GAPDH |
| R&D Biosystems | MAB3626 | Mouse anti-IL-33 |
| R&D Biosystems | MAB006 | Rat IgGA Isotype control |
∗
DRA antibody was raised against the C-terminal amino acid (745–764) sequence: INTNGGLRNRVYEPVETKF of SLC26A3 (accession number: BC025671) at Pocono Rabbit Farms, Canadensis, PA and at UIC Research Resource Center.