Table 4.
Immune Assays.
| Study | DTH/ELISPOT assays | ICS | Other immune assays? | Phenotypic analysis? | |||
|---|---|---|---|---|---|---|---|
| Assay | Stimulus | Positive responses? | Correlation with clinical outcomes? | ||||
|
Oh (2020) Oh (2016) |
ELISPOT | Whole Cell | Yes | NR | No | No | No |
| Bota (2018) | Neither | NA | NA | NA | No | CD3/CD4 + count; maximal and end of treatment | Yes |
| Schulze (2009) | Neither | NA | NA | NA | No | No | No |
| Peng (2006) | DTH | Lysate | Yes | Yes | No | No | No |
| Uyl-de Groot (2005), Vermorken (1999) | DTH | Whole Cell | No | NA | No | No | No |
| Peng (2005) | DTH | Lysate | Yes | Yes | No | Flow cytometry; proportion of CD3, CD4, CD8, CD16, and CD56 positive cells | Yes |
| Kuang (2004) | DTH | Lysate | Yes | Yes | No | No | No |
| Harris (2000) | DTH | Whole Cell | Yes | Yes | No | No | No |
| Galligioni (1996) | DTH | Whole Cell | Yes | NR | No | No | No |
| Hoover (1993) | DTH | Whole Cell | Yes | NR | No | No | No |
| Gray (1989, 1988) | Neither | NA | NA | NA | No | No | No |
| Adler (1987) | DTH | Whole Cell | Yes | Yes | No | In vitro lymphocyte stimulation, immune response to tuberculin | No |
| Souter (1981) | Neither | NA | NA | NA | No | No | No |
| Embleton (1978), McIllmurray (1977) | Neither | NA | NA | NA | No | In vitro cytotoxicity and leukocyte responses to stimulants PHA, PWM, ConA | No |
DTH delayed type hypersensitivity, ICS intracellular cytokine staining, ELISPOT enzyme linked immunospot, NR not reported, NA not applicable, PHA phytohaemagglutinin, PMW pokeweed mitogen, ConA concanavalin A.