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Identification of a peptide modified with OPP at the C terminus.A, tandem mass spectrum of the canonical tryptic peptide TLVLSNLSYSATEETLQEVFEK (MH3+ at 834.4269) from Nucleolin (NCL). B, tandem mass spectrum of a truncated form of this peptide (MH3+ at 859.0979) modified with OPP at the C terminus. Peaks marked with asterisks represent losses of H2O or NH3 from the main sequence ions. OPP, O-propargyl-puromycin.
