FIG. 6.

Metabolic pathways for formation of AFG₁ from OMST and formation of AFG₂ from DHOMST. At least three enzyme reactions may be commonly present in both pathways. Reaction 1 may be catalyzed by an ordA gene product, which is microsome cytochrome P-450 oxygenase. A transient intermediate formed during this pathway may be subsequently converted to another substance in reaction 2 and finally converted to the final product, AFG₁ or AFG₂, in reaction 3. Reactions 2 and 3 may be catalyzed by an unstable microsome enzyme and a 220-kDa cytosol protein. However, the order of the three enzyme activities was not determined in this study.