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. 2023 Feb 27;9(1):2. doi: 10.1038/s41514-023-00099-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Representative immunohistochemistry micrographs of young and aged C57BL/6 mice lacrimal glands stained with fluorescent-conjugated avidin (Texas Red), capturing degranulating mast cell in the lacrimal gland of aged mice (scale bar, 10 µm). b Bar chart depicting tryptase levels in the lacrimal gland (LG) lysates of young and aged mice. c Bar chart quantifying levels of tryptase in ocular surface tear wash. Ocular surface tear wash of young and aged mice was collected, and mast cell activation was assessed by measuring the levels of tryptase. d Bar chart depicting levels of IL33 in the lacrimal gland lysates of young and aged mice. Cumulative data (mean ± SD) from three independent experiments are shown, with each experiment consisting of n of 4 to 6 mice/group. **p < 0.01, ***p < 0.001.