Table.

Basic principles of the 2 clot lysis time assays.

	Aarhus assay [12]	Groningen assay [15]
Material	Citrated plasma	Citrated plasma
Coagulation trigger	Tissue Factor (Dade Innovin, Siemens Healthcare), 1:5000 dilution	Tissue Factor (Dade Innovin, Siemens Healthcare), 1:1000 dilution
Calcium chloride	26.7 mM	17 mM
Phospholipids	4 μM (Rossix)	10 μM (Avanti Polar Lipids)
Fibrinolysis activator	tPA (Calbiochem, Sigma-Aldrich, Merck), 116 ng/mL. Activity: ≥300,000 IU/mg protein	tPA, (Actilyse, Boehringer Ingelheim), 56 ng/mL. Activity: 522,000 to 696,000 IU/mg protein
Buffer	HEPES buffer 20 mM, NaCl 150 mM, pH 7.4 (Ampliqon)	HEPES buffer 25 mM, 137 mM NaCl, 3.5 mM KCl, 0.1% BSA, pH 7.4
Maximum assay time	80 min	180 min
Plasma dilution (plasma:mix)	1:1.15	1:1
Reader and software	Victor Reader X4; 2030 Work Out and WorkOut 2.5 (Perkin Elmer)	Spectramax 340 kinetic microplate reader and SoftMax Pro (Molecular Devices Corporation), LysisAnalyzer version 4.0.0.0
Parameters	Time from max absorbance to 50% lysis (s); lag phase (s); peak absorbance (AU); time to peak absorbance (s); area under curve (AU×s)	CLT (min); t1/2 coagulation (min); t1/2 fibrinolysis (min); OD max (OD); time to OD max (min); slope ½ coagulation; slope ½ fibrinolysis

Concentration and dilution are the final concentration or dilution in the 96-well microtiter plate.

AU, absorbance units; BSA, bovine serum albumin; CLT, clot lysis time; HEPES, (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid); OD, optical density; tPA, tissue plasminogen activator.