Figure 3.

Diminished interaction between the predicted truncated KASH5 and SUN1. (A) Immunofluorescence staining using the anti-FLAG antibodies in HEK293T cells transfected with plasmids expressing FLAG-KASH5-WT or FLAG-KASH5-MUT proteins. Scale bars, 10 µm. (B) Co-immunoprecipitation using the anti-FLAG antibody with HEK293T cells transfected with plasmids expressing GFP-SUN1 proteins and plasmids expressing FLAG-KASH5-WT or FLAG-KASH5-MUT proteins, followed by western blotting analyses using anti-GFP or anti-FLAG antibodies. Cells transfected with GFP-empty plasmids serves as a negative co-IP control. Arrows indicate the bands corresponding to the proteins of interest.