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. 2021 Jun 17;5:42. Originally published 2020 Mar 3. [Version 2] doi: 10.12688/wellcomeopenres.15748.2

Figure 4. Measurement of stimulation-dependent surface markers using flow cytometry corroborates RNA-Seq data.

Figure 4.

Histograms (left) and normalised geometric mean fluorescence intensity (gMFI) values (right) for Jurkat and total primary CD4+ T cells stained with anti-BTLA, anti-CTLA4, anti-CD86, anti-CD69, or anti-CD25 antibodies in the resting state or following incubation for 2–24 h. gMFI values are the mean ± std. dev. of 3 independent experiments with 3 different cell donors. Values are normalised to the raw value for resting primary T cells in each experiment (set at 1). Histograms are representative plots from one donor.