Table 1. Gene Ontology (GO) terms associated with significantly upregulated genes in stimulated Jurkat and primary T cells.
Top 20 (6 for Jurkat) most significant GO terms associated with genes upregulated in Jurkat, primary memory CD4 + T cells, or both stimulated for 2 h. Redundant terms have been removed for clarity. A full list of significant terms is available in the Extended Data (Dataset S4).
| Cell types | GO Term | Description | P-value |
|---|---|---|---|
| Memory
CD4+ and Jurkat (2 h) |
GO:0042110 | T cell activation | 6.79E-15 |
| GO:1903037 | Regulation of leukocyte cell-cell adhesion | 1.63E-12 | |
| GO:0071356 | Cellular response to tumor necrosis factor | 3.37E-12 | |
| GO:1902105 | Regulation of leukocyte differentiation | 4.86E-12 | |
| GO:0002683 | Negative regulation of immune system process | 2.51E-11 | |
| GO:0001818 | Negative regulation of cytokine production | 2.90E-09 | |
| GO:0001819 | Positive regulation of cytokine production | 5.60E-07 | |
| GO:0032496 | Response to lipopolysaccharide | 6.92E-07 | |
| GO:0051348 | Negative regulation of transferase activity | 1.40E-06 | |
| GO:0071901 | Negative regulation of protein serine/threonine kinase activity | 1.42E-06 | |
| GO:0045444 | Fat cell differentiation | 1.65E-06 | |
| GO:0001503 | Ossification | 6.61E-06 | |
| GO:0032609 | Interferon-gamma production | 6.61E-06 | |
| GO:1904035 | Regulation of epithelial cell apoptotic process | 7.33E-06 | |
| GO:0097191 | Extrinsic apoptotic signaling pathway | 1.16E-05 | |
| GO:1901652 | Response to peptide | 1.54E-05 | |
| GO:0051090 | Regulation of DNA binding transcription factor activity | 2.03E-05 | |
| GO:0051051 | Negative regulation of transport | 2.35E-05 | |
| GO:0002699 | Positive regulation of immune effector process | 2.62E-05 | |
| GO:0060759 | Regulation of response to cytokine stimulus | 6.39E-05 | |
| Memory
CD4+ only (2 h) |
GO:0032496 | Response to lipopolysaccharide | 2.53E-10 |
| GO:0071216 | Cellular response to biotic stimulus | 4.02E-09 | |
| GO:0050731 | Positive regulation of peptidyl-tyrosine phosphorylation | 4.02E-09 | |
| GO:0042110 | T cell activation | 1.07E-08 | |
| GO:0022407 | Regulation of cell-cell adhesion | 3.92E-08 | |
| GO:0001503 | Ossification | 3.92E-08 | |
| GO:0042035 | Regulation of cytokine biosynthetic process | 4.91E-08 | |
| GO:0030099 | Myeloid cell differentiation | 3.91E-07 | |
| GO:1902895 | Positive regulation of pri-miRNA transcription by RNA polymerase II | 3.45E-06 | |
| GO:0002822 | Regulation of adaptive immune response based on somatic recombination
of immune receptors |
3.52E-06 | |
| GO:0032944 | Regulation of mononuclear cell proliferation | 3.52E-06 | |
| GO:0070555 | Response to interleukin-1 | 3.52E-06 | |
| GO:1902105 | Regulation of leukocyte differentiation | 9.17E-06 | |
| GO:0042254 | Ribosome biogenesis | 1.87E-05 | |
| GO:0090287 | Regulation of cellular response to growth factor stimulus | 1.87E-05 | |
| GO:0001818 | Negative regulation of cytokine production | 1.87E-05 | |
| GO:0007178 | Transmembrane receptor protein serine/threonine kinase signaling pathway | 2.67E-05 | |
| GO:0002699 | Positive regulation of immune effector process | 2.71E-05 | |
| GO:0016074 | snoRNA metabolic process | 2.71E-05 | |
| GO:0048608 | Reproductive structure development | 3.38E-05 | |
| Jurkat
only (2 h) |
GO:0042326 | Negative regulation of phosphorylation | 0.004101547 |
| GO:0050804 | Modulation of chemical synaptic transmission | 0.004101547 | |
| GO:0099177 | Regulation of trans-synaptic signaling | 0.004101547 | |
| GO:0022407 | Regulation of cell-cell adhesion | 0.005367298 | |
| GO:0001933 | Negative regulation of protein phosphorylation | 0.007798348 | |
| GO:0001708 | Cell fate specification | 0.0094938 |