Table 1.
Primers used for evaluation of the gene expression by relative quantitative real-time PCR
| Gene | Sequences | Product size (bp) |
|---|---|---|
| MDM2 | 5΄-AACCACCTCACAGATTCCA-3΄ 5΄-GCACCAACAGACTTTAATAACTTC-3΄ |
87 |
| PSMD10 | 5΄-CTACTAGAACTGACCAGGACA-3΄ 5΄-GCCGCAATATGAAGAGGAG-3΄ |
145 |
| SIRT3 | 5΄-ACTCCCATTCTTCTTTCACAAC-3΄ 5΄-GGATGCCCGACACTCT-3΄ |
176 |
| USP7 | 5΄-TGGTGGAGCGATTACAAGA-3΄ 5΄-TCCTCTGCGACTATCTGC-3΄ |
100 |
| Beta-actin [ 18 ] | 5΄-GCCTTTGCCGATCCGC-3΄ 5΄-GCCGTAGCCGTTGTCG-3΄ |
90 |
| TP53 | 5΄-GATAGCGATGGTCTGGC-3΄ 5΄-CGGCTCATAGGGCACC-3΄ |
117 |
| HDAC1 | 5΄-GACGGTAGGGACGGGAG-3΄ 5΄-GGCTTTGTGAGGGCGATAG-3΄ |
203 |
| EBNA1 | 5΄-GGGTGGTTTGGAAAGCATCG-3΄ 5΄-CTTACTACCTCCATATACGAACACA-3΄ |
156 |
| MDM4 | 5΄-GCCTGCCTTGGTGGTT-3΄ 5΄-CCTAACTGCTCTGATACTGACTC-3΄ |
160 |