Table 3.
HPLC purified PAH minisequencing primers
| Primer name | SNP | Sequence (5'-3') |
Primer
length (nt) |
Product
size (bp) |
Concentration
(µM) |
Tm
(°C) |
Gc (%) |
|---|---|---|---|---|---|---|---|
| Set-1 | rs62507288 | F: GCGCTTATTTGAGGTCA | 17 | 17 | 0.02 | 50 | 47 |
| Set-2 | rs62507341 | F: acgtcgtgaaagtctgacaaTCCTCACC CTCCCCA | 15 | 35 | 0.03 | 52 | 67 |
| Set-3 | rs5030843 | F: gccacgtcgtgaaagtctgacaaTGTGTA CCGTGCAAGAC | 17 | 40 | 0.06 | 52 | 53 |
| Set-4 | rs5030849 | R: gacaaGTGTGCAGTGGAAGACT | 17 | 22 | 0.04 | 51 | 53 |
| Set-5 | rs62508637 | F: aggtgccacgtcgtgaaagtctgacaaGAA AAGCTCGCCACAGTAA | 19 | 46 | 0.01 | 52 | 44 |
| Set-6 | rs5030857 | F: aactgactaaactaggtgccacgtcgtgaaagt ctgacaaGGTTTTGGTCTTAGGAACTTTG | 22 | 62 | 0.1 | 52 | 41 |
| Set-7-1 | rs5030855 | F: actaggtgccacgtcgtgaaagtctgacaaT AACAGCGATAATAACTTTTCACTT | 25 | 55 | 0.06 | 52 | 28 |
| Set-7-2 | rs62516095 | R: aactgactaaactaggtgccttttttttttacgtcgtg aaagtctgtgaaagtctgacaaTTTGGCATCTCTGATAAGCA | 20 | 80 | 0.4 | 52 | 40 |
| Set-8-1 | rs199475575 | R: aactgactaaactaggtgccacgtcgtgaaagtct gtgaaagtctgacaaTTCCTCCATGTATTCCACTC | 20 | 70 | 0.13 | 51 | 45 |
| Set- 8-2 | rs5030845 | R: gtctgacaaACTTACTCTGCAGGAA TTGAG | 21 | 30 | 0.06 | 52 | 43 |
F, forward primer; R, reverse primer