Figure 2.

Study of mRNA-B11-LNP prophylactic effect. (A) Schedule of passive B11-mRNA-LNP immunization before BoNT/A challenge. A total of three mice per group were challenged and experiment was performed twice. Mice received a single intramuscular (i.m.) injection of 10 μg of B11-mRNA-LNP or recombinant B11-Fc antibody 0, 3.5, 8 or 24 h before 5 LD₅₀ intraperitoneally injected BoNT/A challenge. Body weight, clinical score and survival were monitored for up to 72 h; (B) Survival curve of mice that were treated according to the schedule in (A). (C, E) Schedules of passive B11-mRNA-LNP immunization (10 μg of B11-mRNA-LNP per mouse) before challenge by different BoNT/A doses (5, 10, 50 and 100 LD₅₀). A total of three mice per group were challenged and experiment was performed twice. Body weight, clinical score and survival were monitored for up to 72 h; (D, F) Survival curves of mice that were treated according to the schedules in (C, E). A total of three mice per group were challenged and experiment was performed twice. LNP-mRNA⁺ – mice that were treated with B11-mRNA-LNP; LNP-mRNA^- – mice that did not receive B11-mRNA-LNP, but were challenged. p-value determined by log-rank (Mantel-Cox) test. “H” on (A, C, E) shows the key points of the experiment chart and means the hours.