TABLE 3.
Substrate specificity of the sMMO from Methylocystis sp. strain WI 14
| Substrate | Product(s) | Sp act (mU/mg) |
|---|---|---|
| Ethylene | Ethylene oxide | 33.6 |
| Propylene | Propylene oxide | 25.4 |
| Butylene | Butylene oxide | 19.1 |
| Propane | 2-Propanol | 334.3 |
| Butane | 2-Butanol | NDb |
| Pentane | n-Pentanola | —c |
| Hexane | n-Hexanol | — |
| Heptane | n-Heptanol | — |
| Diethylic ether | Ethanol, acetaldehyde | 205.7 |
| Cyclohexane | Cyclohexanol | ND |
| Benzene | Phenol | ND |
| Toluene | Cresola | — |
| Xylene | Xylenola | — |
| Naphthalene | Naphthol | 1.2 |
| Chloropentane | Chloropentanol | 3.9 |
| Chlorobenzene | Chlorophenola | — |
| Fluorobenzene | Fluorophenola | — |
| Bromobenzene | Bromophenola | — |
| Chloronaphthalene | Chloronaphthol | 0.8 |
a
The position of hydroxylation could not be determined.
b
ND, not determined.
c
—, determination of the specific activity was not possible because the products were not exactly identified.