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. 2023 Feb 28;16:78. doi: 10.1186/s13071-023-05705-4

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Specificity and LOD (limit of detection) of the Bulinus truncatus eLAMP (Bt-eLAMP) assay. Results obtained with agarose gel electrophoresis after 45 min of reaction. a Specificity; lanes 1–5, Bulinus truncatus DNA templates from five different localities (Diama, Lampsar, Mbane, Corsica and Saneinte); lane 6, Bulinus globosus; lane 7, Bulinus senegalensis; lane 8, Bulinus forskalli; lane 9, Bulinus umbilicatus; lane 10, Biomphalaria glabrata; lane 11, Bithynia tentaculata; lane 12, Ancylus fluviatilis; lane 13, Gyraulus laevis; lane 14, Physa acuta; lane 15, Potamopyrgus antipodarum; lane 16, Pisidium casertanum; lane C + , positive control (Bulinus truncatus DNA; 1 ng); lane C-, negative control (water as template). b LOD; lane 1–11, Bulinus truncatus DNA tenfold serial dilutions (10 ng/μL to 1 ag/μl); lane C-, negative control (water as template)