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. 2023 Feb 28;16:78. doi: 10.1186/s13071-023-05705-4

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Comparison of Bulinus truncatus environmental DNA detection by loop-mediated isothermal amplification (LAMP), quantitative polymerase chain reaction (qPCR) and droplet digital polymerase chain reaction (ddPCR) amplification methods. Each cell represents DNA amplification results from eDNA collected in two Corsican rivers (Cavu and Solenzara) and Perpignan Channel; Bulinus truncatus snails are present in all sites except the water intake site in the Cavu River and university site in the Perpignan Channel. Three volumes of water (1, 3 and 5 l) from both the shore and the streambeds of the rivers were collected in all Corsican sites. Each line represents the amplification result from each site using LAMP, qPCR or ddPCR amplification method. qPCR and ddPCR results come from Mulero et al. [21]. A green membrane means that at least one quarter of the filtration membrane is positive for Bulinus truncatus presence and red membrane means that no quarter of the filtration membrane is positive. Empty cells mean that no filtration was done under these conditions