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. 2023 Feb 28;16:78. doi: 10.1186/s13071-023-05705-4

Table 2.

Primer sequences designed for Bulinus truncatus eLAMP assays

Target Primers Sequences (5’-3’) Sequence length (bp) Melting temperature (°C)
Internal transcribed spacer 2 (ITS2) F3 GTCCTTCCTGTCCCCCTT 18 59.79
B3 CGTCACATGTCCACCTCACA 20 61.59
F2 ACGCATGTCGCCGTTAC 17 59.33
F1c AAGAGCACGAGCCGGGTCC 19 66.28
B2 CGCGTTATAGCCCATCTCAA 20 59.37
B1c GCGAGTCATACGGACCTCGTG 21 64.21
LB CACAGTCTCTCCCCCACACC 20 63.67
LF CCTGGCGACGAGAGATCACC 20 63.89