Table 2.
Primary Antibodies Used for IF, IHC-P, IHC-Fr, and WB
| Antibody | Species | Company | Catalog no. | Dilution |
|---|---|---|---|---|
| α-SMA (IHC-P) | Rabbit anti-mouse | Abcam | ab124964 | 1:200 |
| α-SMA (IHC-Fr) | Goat anti-mouse | Novus | NB300-978 | 1:500 |
| CD68 (IHC-P/Fr) | Rabbit anti-mouse | ThermoFisher | PA5-78996 | 1:500 |
| CD3 (IHC-P) | Rabbit anti-mouse | Abcam | ab21703 | Read to use |
| YM-1 (IHC-P) | Rabbit anti-mouse | Stem cell Technologies | 60130 | 1:50 |
| Ki67 (IHC-P) | Rabbit anti-mouse | Abcam | ab21700 | Ready to use |
| Ki67 (IF) | Rabbit anti-human | Abcam | ab21700 | Ready to use |
| FAP (IHC-P) | Sheep anti-human | R&D | AF3715 | 1:15 |
| GFAP (IHC-Fr) | Mouse anti-mouse | Sigma-Aldrich | G3893 | 1:400 |
| Desmin (IHC-Fr) | Rabbit anti-mouse | ThermoFisher | PA5-16705 | 1:500 |
| Collagen type I (IHC-Fr) | Rabbit anti-mouse | ThermoFisher | PA5-95137 | 1:500 |
| CD68 (IHC-P) | Mouse anti-human | MAIXIN. BIO | MAB-0863 | Ready to use |
| α-SMA (IHC-P) | Mouse anti-human | MAIXIN. BIO | MAB-0890 | Ready to use |
| α-SMA (WB) | Rabbit anti-human | Abcam | ab5694 | 1:1000 |
| Collagen type I (WB) | Rabbit anti-human | ThermoFisher | PA5-95137 | 1:1000 |
| FLAG (WB) | Mouse anti-human | CST | 8146 | 1:1000 |
| GAPDH (WB) | Rabbit anti-human | Abcam | ab9485 | 1:5000 |
α-SMA, α-smooth muscle actin; FAP, fibroblast activation protein; IF, immunofluorescence; IHC-Fr, immunohistochemistry -frozen sections; IHC-P, immunohistochemistry -paraffin sections; WB, Western blot.