Figure 5.

Time-invariant methylated positions. (A), The ICCs stratified by probe type, with QC GV representing the aforementioned quality control probes that bind GVs, the potential GV representing probes that were annotated with a genetic variant, and predicted GV representing probes with a methylation signal typically found when driven by a genetic variant. Red dashed lines represent the classification boundaries introduced by Koo and Li,⁴⁷ with blocks representing poor (ICC < 0.5), moderate (0.5 ≤ ICC < 0.75), good (0.75 ≤ ICC < 0.9), and excellent (0.9 ≥ ICC). (B), Jittered visualisation of the 11 probes that present a time in variant difference that is as stable as the aforementioned quality control probes with the percentage methylation on the y-axis and the time point on the x-axis. Dashed lines connect samples obtained from the same donor. The cross-bar visualization represents the mean and standard error of the mean.