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. 2023 Feb 6;13:1062424. doi: 10.3389/fonc.2023.1062424

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Copyright © 2023 Jin, Huo, Fan, Wang, Scott, Pizzi, Yao, Shao, Ma, Da Silva, Yamashita, Yoshimura, Zhang, Wu, Wang, Song and Ajani

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence (IF) staining of cancer markers KRT19 (CK19) and EpCam, oncogene Yap1, stem cell marker Sox9, EMT marker Vimentin, and fibroblast marker FAP. (A) IF staining in human/mouse mixed pool GA0825 cells (6/13/2019) derived from GA0825-PDX-G1 ( Figure 4E ), human H0825 cells and murine M0825 cells. (B) mCherry labeled murine P0825 was stained with the same markers as in (A), but second antibodies GFP⁴⁸⁸-conjugated Alex Fluor were used for green channel.