Table 3.

Prevalence of Wolbachia in dogs positive for Dirofilaria immitis divided according to the microfilaria load and the range of C_t value of qPCR

Groups		Knott test	n dogs	qPCR Wolbachia
		mfs load		Ct = 0	Prevalence (%); 95% CI	25 ≤ Ct ≤ 30	Prevalence (%); 95% CI	30 < Ct ≤ 34	Prevalence (%); 95% CI	Ct > 35	Prevalence (%); 95% CI
G1	Dim + /Leis +	5 ≤ low ≤ 200	18	7	38.9; 0.173–0.643	2d	11.1; 0.014–0.347	4	22.2; 0.064–0.476	5	27; 0.097–0.535
		200 < medium ≤ 300	10	3	30; 0.067–0.652	3	30; 0.067–0.652	1	10; 0.003–0.445	3	30; 0.067–0.652
		High ≥ 600	19	0	–	12c,d	63.2; 0.384–0.837	5c	26.3; 0.091–0.51	2c	30; 0.067–0.652
	Tot		47	10	21.3; 0.107–0.357	17	36.2; 0.227–0.515	10	21.3; 0.107–0.357	10	21.3; 0.107–0.357
G2	Dim + /Leis−	1 ≤ low ≤ 200	20	5	25%; 0.087–0.491	5	25; 0.087–0.491	6	30; 0.119–0.543	4	20; 0.057–0.437
		200 < medium ≤ 300	11	0	–	5	54.5; 0.234–0.833	6	54.5; 0.234–0.833	0	0
		High ≥ 400	15	0	–	8e	53.3; 0.266–0.787	6e	40; 0.163–0.677	1e	6.7; 0.002–0.319
	Tot		46	5	10.9; 0.036–0.236	18	39.1; 0.251–0.546	18	39.1; 0.251–0.546	5	10.9; 0.036–0.236
Total			93	15	16.1; 0.093–0.252	35a	37.6; 0.278–0.483	28b	30.1; 0.210–0.405	15a,b	16.1; 0.093–0.252

C_t = threshold value, 95% CI = 95% confidence level. Statistically significant differences in prevalence are marked with equal superscript letters. P-value < 0.05