Table 1.

Characterization and validation.

Classification	Test	Result	Data
Morphology	Photography Bright field	Normal	Fig. 1 panel F
Phenotype	Qualitative analysis (Immunocytochemistry)	Staining/expression of pluripotency markers: Oct3/4, Nanog, Sox2, Tra1-60, Tra1-80.	Fig. 1 panel A
	Quantitative analysis (RT-qPCR)	Expression of DNMT3B, NANOG, POU5F1 and SOX2	Fig. 1 panel B
Genotype	HumanCytoSNP-12 array	Resolution 72 kb, no major copy number variations	Fig. 1 panel D
Identity	HumanCytoSNP-12 array OR	> 99.9 % identical SNPs	Table 3
	STR analysis	N/A	N/A
Mutation analysis (IF APPLICABLE)	Sequencing	Hemizygous BGN c.776G > T	Fig. 1 panel C
	Southern Blot OR WGS	N/A	N/A
Microbiology and virology	Mycoplasma	Negative	Supplementary Fig. 2, Supplementary Fig. 3
Differentiation potential	Trilineage differentiation	Expression of appropriate markers of the respective germ layers, i.e. ectoderm, mesoderm and endoderm.	Fig. 1 panel E
List of recommended germ layer markers	Expression of these markers has to be demonstrated at mRNA (RT PCR) or protein (IF) levels, at least 2 markers need to be shown per germ layer	Endoderm: CXCR4, FOXA2, SOX17 Mesoderm: NKX2.5, αSMA (ACTA2), HAND1 Ectoderm: HES5, MAP2, PAX6	Fig. 1 panel E
Donor screening (OPTIONAL)	HIV 1 + 2 Hepatitis B, Hepatitis C	N/A	N/A
Genotype additional info (OPTIONAL)	Blood group genotyping	N/A	N/A
	HLA tissue typing	N/A	N/A