FIG. 3.
Effect of temperature on the activity of the 47-kDa protease. (A) The enzyme (∼2.1 μg) was incubated in 600 μl of Tris buffer containing 5 mM MgCl2 and 1% azocasein for 30 min at various temperatures, and caseinolytic activity was measured as described. The values obtained at 37°C were taken as 100%. Relative activities are the averages for two independent experiments. (B) Arrhenius plot showing thermal deactivation of the 47-kDa protease. The ln of specific activity (k) (100 × EU) was plotted against the reciprocal of absolute temperature (T).