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Purification of Y. ruckeri 47-kDa caseinolytic protease
| Purification step | Total protein (mg) | Total activity (EU) | Sp act (EU/mg) | Purifi-cation (fold) | Yield (%) |
|---|---|---|---|---|---|
| Culture supernatant | 4,400 | 412,533 | 92.91 | 1 | 100 |
| Ammonium sulfate | 11.44 | 176,800 | 154.54 | 1.66 | 42.8 |
| DEAE-Sephacel | 1.12 | 8,580 | 7,633.45 | 82.15 | 2.07 |
