Fig. 1.
Identification of a novel spore protein in Bacillus anthracis. Bacillus anthracis coat proteins from the wild-type Sterne strain were extracted from spores, subjected to 16% SDS-PAGE and stained with Coomassie Blue G-250. The band indicated by an arrowhead was cut out and analyzed using matrix-assisted laser desorption/ionization time-of-flight MS. The peptide sequence is aligned with the best-fit ORF (BAS1956) from the annotated genome of the Sterne strain (inset). Molecular weight markers are indicated in kDa.