Table 1.
Strains and plasmids
| Strain | Genotype/description | Sources |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | Cloning host | Lab stock |
| GM2163 | dam dcm; for transformation of B. anthracis | Lab stock |
| GM1684 | dam | T. Koehler |
| Sterne strains | ||
| 34F2 | pXO1+ pXO2−, wild type | P. Jackson |
| RG61 | pXO1+ pXO2−, cotβ∷km | This study |
| RG134 | pXO1+ pXO2−, cotβΩpAS5 | This study |
| MGM68 | pXO1+ pXO2−, cotβ∷km | This study |
| RG56 | pXO1+ pXO2−, cotE∷km | Giorno et al. (2007) |
| MGM37 | pXO1+ pXO2−, cotE∷km, cotβΩpAS5 | This study |
| Ames strains | ||
| Ames | pXO1+ pXO2+, wild type | Little & Knudson (1986) |
| Ames-JAB-7 | pXO1+ pXO2+, cotβ∷km | This study |
| Ames-JAB-10 | pXO1+ pXO2+, cotβΩpEO3-cotβ-gfp | This study |
| Name | Description | Sources |
| Plasmids | ||
| pMR1 | Used to introduce kanr into the chromosome by marker replacement | Giorno et al. (2007) |
| pMR2 | Used to replace cotβ with kanr | This study |
| pAS1 | Used to introduce gfp-fusion genes into the chromosome | Lab stock |
| pAS5 | pAS1 bearing 3′ fragment of cotβ | This study |
| pEO3 | E. coli/Bacillus shuttle vector | Mendelson et al. (2004) |
| pJRS182 | Origin of the Ωkm-2 cassette | Perez-Casal et al. (1991) |
| pJRS100.2 | Source of the Ωkm-2 cassette | M. Caparon |
| pEO3-cotβ | pEO3+1.3 kb fragment containing cotβ | This study |
| pEO3-cotβ-Ωkm-2 | pEO3-cotβ bearing Ωkm-2 | This study |
| pEO3-cotβ-gfp | pEO3 containing cotβ-gfp | This study |