FIGURE 2:

Activation of PPARγ-dependent transcription by regioisomers of NO₂-LA., The inset shows a Western blot of PPARγ expression in MCF7/RTO/PPARG1–4ʹ cells grown in the presence (+) or absence (−) of doxycycline. The bar graph shows the induction of PPRE-containing reporter gene expression by NO₂-LA isomers in the absence (light gray bars, with doxycycline) or presence (dark gray bars, without doxycycline) of PPARγ transgene expression. Induction was accomplished with 2 µM NO₂-LA isomer or vehicle (control) as described in Experimental Procedures. Fold induction is defined as (corrected PPRE reporter activity + inducing agent)/[corrected PPRE reporter activity − inducing agent in doxycycline-treated (−PPARγ) cells]. Bars represent the mean values of triplicate determinations ± one standard deviation.