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. 2023 Feb 23:eadf0348. doi: 10.1126/sciimmunol.adf0348

Fig. 3. MPC inhibition dampens HIF-1α levels in lung macrophages.

Fig. 3.

(A to C) Immunoblot analysis of indicated total or phosphorylated protein levels in mouse AMs (A) and BMDM (C) stimulated with or without Poly IC in the presence of vehicle or MSDC in vitro. (B) Quantification of HIF-1α levels in (A) of three experiments were shown. (D) Flow cytometry analysis of HIF-1α levels in AMs from naïve or IAV-infected wt mice treated with vehicle or MSDC at 4 d.p.i. (n = 5). (E) GSEA of hypoxia gene set shown for Poly IC stimulated AMs with vehicle or MSDC treatment. (F) Immunoblot analysis of HIF-1α in human AMs stimulated with or without Poly IC in the presence of vehicle or MSDC overnight in vitro (left). Quantitation on the right (n = 3 donors). (G) Ccl2 and Tnf levels in mouse AMs under indicated conditions overnight in vitro. Representative immunoblots (A, C and F) were from three independent experiments. Data are presented as means ± SEM. *, p < 0.05. The p value was determined by a two-tailed Student’s t-test (B, D, and F) and one-way ANOVA (G).