FIG 10.

ASFV-ΔH240R infection induces higher inflammatory cytokine production in pigs than its parental ASFV HLJ/18. (A to C) Analysis of inflammatory cytokines in tissues and serum from ASFV-WT- and ASFV-ΔH240R-challenged pigs. Pigs were inoculated with PBS (control [ctrl], n = 4) or challenged intramuscularly with ASFV-ΔH240R (n = 6, 10³ and 10⁵ HAD₅₀) or parental ASFV-WT (n = 6, 10³ HAD₅₀). At 21 dpi, the surviving pigs were euthanized, and tissues including spleen, tonsil, and mediastinal lymph node were collected from all pigs to detect the Tnf-α mRNA level by qPCR (A). The protein levels of IL-1β (B) and TNF-α (C) in the serum from ASFV-WT- and ASFV-ΔH240R-challenged pigs were measured by ELISA. (D to E) Analysis of antibody in serum from ASFV-WT- and ASFV-ΔH240R-challenged pigs. At 0, 1, 4, 7, 11, 16, and 21 dpi, the antibodies against p30 (D) and p54 (E) in the serum samples from ASFV-WT- and ASFV-ΔH240R-challenged pigs were measured by ELISA. The data presented are the mean ± SD. *, P < 0.05; **, P < 0.01.