FIG 2.

Experimental design and effects of substrate raising conditions on ANG development. Squid raised in Connecticut under “lab sand” conditions: Hatchlings were first raised with autoclaved sand and filter-sterilized artificial seawater until they were approximately 8–10 mm mantle length. They were then transferred to tanks that had previously housed wild-caught squid on unautoclaved sand and raised to sexual maturity. For the “wet-collected conditions,” squid were raised on sand obtained from below the low-tide mark in Maunalua Bay, Oahu, HI. Squid were also raised on wet-collected sand that had been autoclaved prior to rearing. In separate experiments, Leisingera sp. JC1 was added to tanks for squid which were also raised on wet-collected sand. For “dry-collected conditions,” squid were raised on substrate collected from above the high-tide mark (see methods), and specific ANG isolates were added to some conditions as shown. Numbers on the right indicate the number of ANGs that formed in adult animals out of the total number of female animals from that condition.